Anti-apoptosis effects of vascular endothelial cadherin in experimental corneal neovascularization

作者：Liu Gao Qin; Wu Hong Ya; Xu Jing; Wang Meng Jiao; Lu Pei Rong^*; Zhang Xue Guang

来源：International Journal of Ophthalmology, 2015, 8(6): 1083-1088.

DOI：10.3980/j.issn.2222-3959.2015.06.01

• vascular endothelial cadherin
• neovascularization
• cornea
• chemokine

AIM: To explore the effects and mechanism of vascular endothelial cadherin (VE -cadherin) on experimental corneal neovascularization (CRNV).
METHODS: Mouse corneas were burned with sodium hydroxide to build a CRNV model. The burned corneas were locally administrated with anti-mouse VE-cadherin neutralizing antibody. Annexin V and cluster of differentiation 31 (CD31) double staining was used to measure vascular endothelial cell apoptosis with the use of flow cytometry (FCM). The protein expression of NADPH oxidase 2 (Nox2), caspase-3, and protein kinase C (PKC) in the burned corneas were examined by Western blot. Human retinal endothelial cell (HREC) proliferation was detected using a Cell Counting Kit 8 (CCK-8) assay in vitro.
RESULTS: The amount of CRNV peaked two weeks after the alkali burn. FCM confirmed that VE -cadherin neutralizing antibody treatment increased CD31 positive cell apoptosis. Western blot revealed that the intracorneal protein expression of Nox2 and caspase -3 were up regulated, while PKC was down -regulated in the VE cadherin neutralizing antibody administrated group. CCK -8 assay showed that VE -cadherin neutralizing antibody markedly inhibited HREC proliferation.
CONCLUSION: VE-cadherin exhibited an anti-apoptosis effect through enhanced PKC signaling and an enhanced cell proliferation pathway.

• 出版日期2015-12-18
• 单位苏州大学