Urinary d-lactate levels reflect renal function in aristolochic acid-induced nephropathy in mice

作者：Huang Tzu Chuan; Chen Shih Ming; Li Yi Chieh; Lee Jen Ai^*

来源：Biomedical Chromatography, 2013, 27(9): 1100-1106.

DOI：10.1002/bmc.2908

• aristolochic acid nephropathy
• renal injury
• d-lactate
• l-lactate
• urine

Urinary D-lactate is highly correlated to diabetic nephropathy - a progressive kidney disease in renal glomeruli. In this study, we used a C3H/3e mouse model to investigate the relationship between urinary D-lactate and aristolochic acid nephropathy where the glomerular structure is not affected. The nephropathy was induced using intravenous injections of aristolochic acid at a dosage of 10 mg/kg per day for 5 days and was characterized biochemically and histologically. The urinary excretions of proteins, N-acetyl-beta-D-glucosaminidase and serum creatinine were determined and connected to histological conventional findings. Urinary D-lactate was analyzed using column-switching high-performance liquid chromatography with fluorescence detection. The results showed a remarkable increase of urinary markers, including of urinary proteins and N-acetyl-beta-D-glucosaminidase, and the histological examination confirmed a diagnosis of acute tubule necrosis. The ratio of D-lactate to creatinine in the urine of aristolochic acid-treated mice was approximately 36 times greater than that of the mice in the control group (p %26lt; 0.05). The ratios for the two groups of mice were 311.00 +/- 71.70 and 8.60 +/- 1.80 mu mol/mmol creatinine, respectively. These data confirm in vivo that urinary D-lactate reflects renal injury conditions in aristolochic acid-treated mice and may be a marker for the assessment of nephropathy.

• 出版日期2013-9