Depuration kinetics of hepatitis A virus in clams

作者：Polo David; Alvarez Cristina; Luz Vilarino Ma; Longa Angeles; Romalde Jesus L^*

来源：Food Microbiology, 2014, 39: 103-107.

DOI：10.1016/j.fm.2013.11.011

• Depuration
• Clam
• Hepatitis A virus
• Real time RT-PCR
• Infectivity

The efficacy and dynamic of depuration for the removal of hepatitis A virus (HAV) contamination were evaluated under experimental conditions using Manila clams previously subjected to bioaccumulation with this virus. Five independent trials were assayed in a closed experimental system with a total volume of approximately 1750 I, using clam batches of 60 Kg. The reverse transcriptase-real time PCR (RT-qPCR) technique was utilized for viral quantification. Infectivity assays were conducted at the end of depuration. Although the final viral loads in shellfish after 7 days remained relatively high and still infectious, an average reduction in HAV levels of 1.44 log units (approx. 93.1%) was observed. This reduction showed a two-phase removal kinetic, with an initial rapid reduction of viruses during the first 72 h of depuration, with a 0.6 log units (69%) of average decrease in HAV RNA copies/g digestive tissue, and a subsequent stabilization with a slower depuration rate in the remaining days.

• 出版日期2014-5