Development of a polymerase chain reaction-restriction fragment length polymorphism assay for Leishmania major/Leishmania killicki/ Leishmania infantum discrimination from clinical samples, application in a Tunisian focus

作者：Haouas Najoua^*; Garrab Selma; Gorcii Mohamed; Khorchani Hassen; Chargui Najla; Ravel Christophe; Mezhoud Habib; Babba Hamouda

来源：Diagnostic Microbiology and Infectious Disease, 2010, 68(2): 152-158.

DOI：10.1016/j.diagmicrobio.2010.06.011

• Leishmaniasis
• Species identification
• PCR-RFLP

Topoisomerase II gene of Leishmania genus was used to develop a molecular tool for detection and species differentiation of Leishmania from clinical samples. Identification was achieved by a polymerase chain reaction followed by digestion with 2 restriction endonucleases BstU1 and Taq1. Despite the relatively low sensitivity, it is able to differentiate between 3 complexes responsible for cutaneous leishmaniasis.

• 出版日期2010-10