Analysis of Cibacron blue F3G-A interaction with therapeutic proteins by MALDI-TOF mass spectrometry

作者：Sutkeviciute Ieva; Sereikaite Jolanta^*; Bumelis Vladas Algirdas

来源：Biomedical Chromatography, 2008, 22(9): 1001-1007.

DOI：10.1002/bmc.1019

• Cibacron blue F3G-A
• recombinant human growth hormone
• recombinant interferon-alpha 2b
• MALDI-TOF mass spectrometry

The formation of the complexes between Cibacron blue F3G-A and two therapeutic proteins, recombinant human interferon-alpha 2b and recombinant human growth hormone, was investigated. The method of time-resolved limited proteolysis coupled with MALDI-TOF mass spectrometry was used. The analysis of peptide maps revealed that A (HR19)-H-17 and L(20)OHQLAFDTYQEFEEAYIIPK(38) of hGH, and R(14)TLNILLAQMR(23) and D(33)RHDFGFPQEEFGNQFQK(50) of hIFN-alpha 2b, exhibit affinity to Cibacron blue F3G-A.

• 出版日期2008-9