Derivatization of Aminoglycoside Antibiotics with Tris(2,6-dimethoxyphenyl)carbenium Ion

作者：Topolyan A P; Belyaeva M A; Bykov E E; Coodan P V; Rogozhin E A; Strizhevskaya D A; Ivanova O M; Ustinov A V; Mikhura I V; Prokhorenko I A; Korshun V A; Formanovsky A A

来源：Acta Naturae, 2016, 8(3): 128-135.

DOI：10.32607/20758251-2016-8-3-128-135

<jats:p>Detection of aminoglycoside antibiotics by MS or HPLC is complicated, because a) carbohydrate molecules have low ionization ability in comparison with other organic molecules (particularly in MALDI-MS), and b) the lack of aromatics and/or amide bonds in the molecules makes common HPLC UV-detectors useless. Here, we report on the application of a previously developed method for amine derivatization with tris(2,6-dimethoxyphenyl)carbenium ion to selective modification of aminoglycoside antibiotics. Only amino groups bound to primary carbons get modified. The attached aromatic residue carries a permanent positive charge. This makes it easy to detect aminoglycoside antibiotics by MS-methods and HPLC, both as individual compounds and in mixtures.</jats:p>

• 出版日期2016-9