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摘要
Purpose: The aim of this study was to analyze the effect of azithromycin (AZM) 1% ophthalmic solution in DuraSite (R) (AzaSite (R)) on biofilm formation by Staphylococcus aureus and coagulase-negative staphylococci in vitro.
Methods: Susceptible and resistant clinical strains (n = 8) of S. aureus and coagulase-negative staphylococci were challenged with serial dilutions of AzaSite (R) and its components: AZM, benzalkonium chloride (BAK), and the DuraSite drug delivery vehicle. After 20 h of incubation, bacterial growth was quantified using a spectrophotometer (A-600 nm). Plates were stained with crystal violet and biofilm formation was quantified spectrophotometrically at A 590 nm.
Results: AzaSite (R) and AZM inhibited bacterial growth (P < 0.05) and biofilm formation (P < 0.05) in AZM-susceptible strains at all studied dilutions. AZM-resistant strains treated with AzaSite (R) exhibited a significant reduction in biofilm formation (P < 0.05) at subinhibitory concentrations (1.25%-5%). AZM had no effect on bacterial growth in resistant strains but conferred a small reduction in biofilm formation at concentrations from 1.25 to 10mg/mL in most strains. DuraSite (R) inhibited biofilm formation at concentrations between 10% and 2.5% in all studied strains (P < 0.05), without affecting bacterial growth. BAK inhibited bacterial growth and biofilm formation in all strains between concentrations of 0.042 and 0.375mg/mL (P < 0.05).
Conclusions: AzaSite (R), AZM, or BAK prevented biofilm formation by inhibiting growth of AZM-susceptible strains. AzaSite (R), AZM, and DuraSite (R) also reduced biofilm formation at subinhibitory concentrations for growth. Our data indicate that AZM has a moderate inhibitory effect on biofilm formation, whereas DuraSite (R) appears to play a greater role in the inhibition of staphylococcal biofilm formation by AzaSite (R).
- 出版日期2010-12
