Modification of lymphocyte DNA damage by carotenoid supplementation in postmenopausal women

作者：Zhao XF; Aldini G; Johnson EJ; Rasmussen H; Kraemer K; Woolf H; Musaeus N; Krinsky NI; Russell RM; Yeum KJ^*

来源：American Journal of Clinical Nutrition, 2006, 83(1): 163-169.

• mixed carotenoids
• lutein
• beta-carotene
• lycopene
• DNA damage
• comet assay
• elderly women

Background: Oxidative stress has been implicated in the pathogenesis of chronic diseases related to aging such as cancer and cardiovascular disease. Carotenoids could be a part of a protective strategy to minimize oxidative damage in vulnerable populations, such as the elderly.
Objective: Our aim was to determine the protective effect of carotenoids against DNA damage.
Design: A randomized, double-blind, placebo-controlled intervention study was conducted. Thirty-seven healthy, nonsmoking postmenopausal women aged 50-70 y were randomly assigned to 1 of 5 groups and were instructed to consume a daily dose of mixed carotenoids (beta-carotene, lutein, and lycopene; 4 mg each), 12 mg of a single carotenoid (beta-carotene, lutein, or lycopene), or placebo for 56 d. Plasma carotenoid concentrations were analyzed by using HPLC, and lymphocyte DNA damage was measured by using a single-cell gel electrophoresis (comet) assay.
Results: At day 57, all carotenoid-supplemented groups showed significantly lower endogenous DNA damage than at baseline (P < 0.01), whereas the placebo group did not show any significant change. Significantly less (P < 0.05) endogenous DNA damage was found as early as day 15 in the mixed carotenoid (P < 0.01) and beta-carotene (P < 0.05) groups.
Conclusions: The results indicate that carotenoid supplementation decreases DNA damage and that a combination of carotenoids (4 mg each of lutein, beta-carotene, and lycopene), an intake that can be achieved by diet, or a larger dose (12 mg) of individual carotenoids exerts protection against DNA damage.

• 出版日期2006-1