Antihypertensive Effects of Silk Fibroin Hydrolysate by Alcalase and Purification of an ACE Inhibitory Dipeptide

作者：Zhou Fengjuan; Xue Zhaohui; Wang Jiehua^*

来源：Journal of Agricultural and Food Chemistry, 2010, 58(11): 6735-6740.

DOI：10.1021/jf101101r

• Silk fibroin
• alcalase protein hydrolysates
• ACE inhibitory peptide
• antihypertensive effect
• spontaneously hypertensive rat

Silk fibroin, which is normally discarded as an industrial byproduct in clothing plants, was hydrolyzed with alcalase. Angiotensin I converting enzyme (ACE) inhibitory activities of the silk fibroin hydrolysates (SFH) were investigated, and the hydrolysate with hydrolysis degree of 17% exhibited the highest ACE inhibitory activity. At the tested 600 mg/kg.d and 1200 mg/kg.d doses, SFH significantly lowered blood pressure of spontaneously hypertensive rats (SHR) after chronic oral administration. SFH was further purified using consecutive chromatographic methods on Sephadex G-15 column and reverse phase high-performance liquid chromatography (RP-HPLC) on an octadecylsilane column. After its purity was confirmed by analytical RP-HPLC and capillary electrophoresis, one ACE inhibitory dipeptide was isolated, and its molecular mass and amino acid sequence were determined as 238.2 Da and Gly-Tyr, respectively, by LC-ESI/MS. The results of this study suggest that silk fibroin byproducts have the possibility to become an effective source for ACE inhibitory peptides.

• 出版日期2010-6-9
• 单位天津大学