Molecularly imprinted polymers with novel functional monomer for selective solid-phase extraction of gastrodin from the aqueous extract of Gastrodia elata

作者：Ji, Wenhua; Chen, Lingxiao; Ma, Xiuli; Wang, Xiao^*; Gao, Qianshan; Geng, Yanling; Huang, Luqi

来源：Journal of Chromatography A, 2014, 1342: 1-7.

DOI：10.1016/j.chroma.2014.03.046

• Molecular imprinted polymers
• Gastrodin
• Solid-phase extraction
• Novel functional monomer

Molecular imprinted polymers (MIPs) with high selectivity and affinity to gastrodin in water were designed using allyl 2,3,4,6-tetra-O-acetyl-glucopyranoside (TAGL) and 1,2,3,4,5-pentafluoro-6-vinylbenzene (PFVB) as novel functional monomers. Binding characterization of pre-polymerization complexes was researched by nuclear magnetic resonance (NMR) and the MIPs were characterized by scanning electron microscopy (SEM). The properties involving adsorption isotherm, adsorption kinetics and selective recognition capacity were evaluated. The MIPs/TAGL exhibited good site accessibility in which it only took 30 min to achieve adsorption equilibrium and highly selective recognition for the template. Furthermore, the performance of the MIPs/TAGL as solid phase extraction material was investigated in detail and hot water at 50 degrees C served as the eluting solvent. Pure gastrodin with the recovery of 76.6% was obtained from the aqueous extract of Gastrodia elata roots.

• 出版日期2014-5-16
• 单位中国中医科学院; 山东农业大学; 山东省科学院