Antimicrobial Activity of the Synthetic Peptide Scolopendrasin II from the Centipede Scolopendra subspinipes mutilans

作者：Kwon Young Nam; Lee Joon Ha; Kim In Woo; Kim Sang Hee; Yun Eun Young; Nam Sung Hee; Ahn Mi Young; Jeong MiHye; Kang Dong Chul; Lee In Hee; Hwang Jae Sam^*

来源：Journal of Microbiology and Biotechnology, 2013, 23(10): 1381-1385.

DOI：10.4014/jmb.1306.06013

• Antimicrobial peptide
• SVM algorithm
• alpha-helical
• Scolopendra subpinipes mutilans

The centipede Scolopendra subpinipes mutilans is a medicinally important arthropod species. However, its transcriptome is not currently available and transcriptome analysis would be useful in providing insight into a molecular level approach. Hence, we performed de novo RNA sequencing of S. subpinipes mutilans using next-generation sequencing. We generated a novel peptide (scolopendrasin II) based on a SVM algorithm, and biochemically evaluated the in vitro antimicrobial activity of scolopendrasin II against various microbes. Scolopendrasin II showed antibacterial activities against gram-positive and -negative bacterial strains, including the yeast Candida albicans and antibiotic-resistant gram-negative bacteria, as determined by a radial diffusion assay and colony count assay without hemolytic activity. In addition, we confirmed that scolopendrasin II bound to the surface of bacteria through a specific interaction with lipoteichoic acid and a lipopolysaccharide, which was one of the bacterial cell-wall components. In conclusion, our results suggest that scolopendrasin II may be useful for developing peptide antibiotics.

• 出版日期2013-10