Accuracy in molecular sexing of martens (Martes americana and Martes caurina) varies among sample types

作者：Campbell Grant M^*; Pauli Jonathan N; Thomas Joshua G; Mcclean Terry

来源：Molecular Ecology Resources, 2010, 10(6): 1019-1022.

DOI：10.1111/j.1755-0998.2010.02856.x

• SRY sequence
• noninvasive sampling
• mesocarnivore

We evaluated the accuracy of sex identification using the SRY marker for American marten (Martes americana) and Pacific marten (Martes caurina) using samples collected from commercial trappers and those obtained via noninvasive sampling. We determined that sex identification from Lut-SRY primers is accurate at > 90% for muscle and hair samples collected noninvasively. We found much lower accuracy when using hair samples plucked from trapper-killed carcasses, errors presumably incurred from contamination from the DNA of trappers, who were entirely male. We also found that the sequence generated from Lut-SRY primers, originally developed for Eurasian otters (Lutra lutra), differed slightly for martens, and recommend that new primers be developed from the sequences we provide. The SRY marker can be reliably used for sex identification in both species of marten, provided that samples have low probabilities of contamination. Researchers should avoid samples collected from external locations on trapper-killed carcasses for DNA-based analyses.

• 出版日期2010-11