Mapping biological to clinical phenotypes during the development (21 days) and resolution (21 days) of experimental gingivitis

作者：Scott Ann E; Milward Mike; Linden Gerard J; Matthews John B; Carlile Monica J; Lundy Fionnuala T; Naeeni Mojgan A; Martin S Lorraine; Walker Brian; Kinane Denis; Brock Gareth R; Chapple Iain L C^*

来源：Journal of Clinical Periodontology, 2012, 39(2): 123-131.

DOI：10.1111/j.1600-051X.2011.01825.x

• biomarkers
• cathepsin G
• experimental gingivitis
• gingival crevicular fluid (GCF)
• interleukin-1 ss
• inflammation
• substance P

Aim: To characterize and map temporal changes in the biological and clinical phenotype during a 21-day experimental gingivitis study. %26lt;br%26gt;Materials and Methods: Experimental gingivitis was induced over 21 days in healthy human volunteers (n = 56), after which normal brushing was resumed (resolution phase). Gingival and plaque indices were assessed. Gingival crevicular fluid was collected from four paired test and contra-lateral control sites in each volunteer during induction (Days 0, 7, 14 and 21) and resolution (Days 28 and 42) of experimental gingivitis. Fluid volumes were measured and a single analyte was quantified from each site-specific, 30s sample. Data were evaluated by analysis of repeated measurements and paired sample tests. %26lt;br%26gt;Results: Clinical indices and gingival crevicular fluid volumes at test sites increased from Day 0, peaking at Day 21 (test/control differences all p %26lt; 0.0001) and decreased back to control levels by Day 28. Levels of four inflammatory markers showed similar patterns, with significant differences between test and control apparent at Day 7 (substance P, cathepsin G, interleukin-1 beta, elastase: all p %26lt; 0.03) and peaking at Day 21 (all p %26lt; 0.002). Levels of alpha-1-antitrypsin showed no pattern. %26lt;br%26gt;Conclusions: Levels of substance P, cathepsin G, interleukin-1b and neutrophil elastase act as objective biomarkers of gingival inflammation induction and resolution that typically precede phenotypical changes.

• 出版日期2012-2