NLRP3 inflammasome activation mediates radiation-induced pyroptosis in bone marrow-derived macrophages

作者：Liu Yan gang; Chen Ji kuai; Zhang Zi teng; Ma Xiu juan; Chen Yong chun; Du Xiu ming; Liu Hong; Zong Ying^*; Lu Guo cai^*

来源：Cell Death & Disease, 2017, 8(2): e2579.

DOI：10.1038/cddis.2016.460

A limit to the clinical benefit of radiotherapy is not an incapacity to eliminate tumor cells but rather a limit on its capacity to do so without destroying normal tissue and inducing inflammation. Recent evidence reveals that the inflammasome is essential for mediating radiation-induced cell and tissue damage. In this study, using primary cultured bone marrow-derived macrophages (BMDM) and a mouse radiation model, we explored the role of NLRP3 inflammasome activation and the secondary pyroptosis underlying radiation-induced immune cell death. We observed an increasing proportion of pyroptosis and elevating Caspase-1 activation in 10 and 20 Gy radiation groups. Nlrp3 knock out significantly diminished the quantity of cleaved-Caspase-1 (p10) and IL-1 beta as well as the proportion of pyroptosis. Additionally, in vivo research shows that 9.5 Gy of radiation promotes Caspase-1 activation in marginal zone cells and induces death in mice, both of which can be significantly inhibited by knocking out Nlrp3. Thus, based on these findings, we conclude that the NLRP3 inflammasome activation mediates radiation-induced pyroptosis in BMDMs. Targeting NLRP3 inflammasome and pyroptosis may serve as effective strategies to diminish injury caused by radiation.

• 出版日期2017-2
• 单位中国人民解放军第二军医大学