Upregulation of stromal cell derived factor-1 alpha in collagen vascular diseases-associated interstitial pneumonias (CVDs-IPs)

作者：Margaritopoulos Giorgos A; Antoniou Katerina M^*; Soufla Giannoula; Karagiannis Konstantinos; Proklou Athanasia; Lasithiotaki Ismini; Tzanakis Nikolaos; Spandidos Demetrios A; Siafakas Nikolaos M

来源：Pulmonary Pharmacology & Therapeutics, 2010, 23(2): 115-120.

DOI：10.1016/j.pupt.2009.10.008

• Angiogenesis
• Connective tissue disorders
• Interstitial pneumonias
• Chemokines
• Pulmonary fibrosis

Objective: We speculated that distinct angiogenic profiles are involved in idiopathic interstitial pneumonias (IIPs) in comparison with interstitial pneumonias associated with collagen vascular disease (CVD-IPs). This hypothesis was investigated by measuring the expression of a cardinal biologic axis, the vascular endothelial growth factor (VEGF)-stromal derived growth factor [SDF-1 alpha, transcripts 1 and 2 (TR1 and TR2)] and receptor, CXCR4 and the angiogenetic receptors CXCR2 and CXCR3 in bronchoalveolar lavage fluid (BALF) in both conditions.
Methods: We studied prospectively 25 patients with fibrotic IIPs (f-IIPs) [20 with idiopathic pulmonary fibrosis (IPF) and 5 with idiopathic non-specific interstitial pneumonia (NSIP)] and 16 Patients with CVD-IPs. mRNA expression was measured by Real-Time RT-PCR and protein was evaluated by Western Blotting.
Results: A significantly greater value has been detected in SDF-1 alpha-TR1 mRNA expression levels of CVD-IPs (p = 0.05) in comparison with IPF group. A similar trend has been also detected in protein expression in favor of CVD-IP group. In addition, VEGF mRNA levels have been found significantly increased in CVD-IPs in comparison with the NSIP group (p = 0.05). No significant difference has been found in SDF-1 alpha-TR2-CXCR4 tnRNA and CXCR2-CXCR3 between the two groups.
Conclusion: These results showed increased expression of SDF-1 alpha in CVD-IPs, suggesting different angiogenic procedures. Further studies are needed in order to better explore the angiogenetic pathway in these disorders.

• 出版日期2010-4