Parentage determination in black tiger shrimp (Penaeus monodon) based on microsatellite DNA markers

作者：Zhu, Kecheng; Yu, Wenbo; Huang, Jianhua; Zhou, Falin; Guo, Huayang; Zhang, Nan; Jiang, Shigui; Zhang, Dianchang^*

来源：Aquaculture International, 2017, 25(2): 827-836.

DOI：10.1007/s10499-016-0082-1

• Penaeus monodon
• Parentage assignment
• Microsatellite markers
• Simulation analysis

Parentage determination was a kind of important technology of selective breeding for aquaculture species. In this study, fourteen microsatellite markers were isolated and chosen to estimate the parentage of 100 progeny in ten black tiger shrimp (Penaeus monodon) full-sib families. Simulation analysis indicated that the power of five loci to exclude false parents was 98.9 % and that of six loci over 99.9 % based on allele frequency data. Moreover, the cumulative assignment success rate was 99 % when no parent information was available using six most informative microsatellite markers. Furthermore, the marker panel tested in mixed families, 89.0 % of progeny was correctly assigned to their parental pairs. Based on the microsatellite analysis, it could be quickly and effectively identified different genealogy and sources of P. monodon; moreover, it provided for selective breeding and multiplication release in this species.

• 出版日期2017-4
• 单位上海海洋大学; 中国水产科学研究院