Embryoid Body-Explant Outgrowth Cultivation from Induced Pluripotent Stem Cells in an Automated Closed Platform

作者：Tone Hiroshi; Yoshioka Saeko; Akiyama Hirokazu; Nishimura Akira; Ichimura Masaki; Nakatani Masaru; Kiyono Tohru; Toyoda Masashi; Watanabe Masatoshi; Umezawa Akihiro^*

来源：Biomed Research International, 2016, 2016: 7098987.

DOI：10.1155/2016/7098987

Automation of cell culture would facilitate stable cell expansion with consistent quality. In the present study, feasibility of an automated closed-cell culture system "P 4C S" for an embryoid body-(EB-) explant outgrowth culture was investigated as a model case for explant culture. After placing the induced pluripotent stem cell-(iPSC-) derived EBs into the system, the EBs successfully adhered to the culture surface and the cell outgrowth was clearly observed surrounding the adherent EBs. After confirming the outgrowth, we carried out subculture manipulation, in which the detached cells were simply dispersed by shaking the culture flask, leading to uniform cell distribution. This enabled continuous stable cell expansion, resulting in a cell yield of 3.1 x 10(7). There was no evidence of bacterial contamination throughout the cell culture experiments. We herewith developed the automated cultivation platform for EB-explant outgrowth cells.

• 出版日期2016