AN EFFICIENT TECHNIQUE FOR OBTAINING SEQUENCES FLANKING INSERTED RETROVIRUSES

作者：HUCKABY CS; KOURI RE; LANE MJ; PESHICK SM; CARROLL WT; HENDERSON SM; FALDASZ BD; WATERBURY PG; VOURNAKIS JN

来源：Genetic Analysis - Biomolecular Engineering, 1991, 8(5): 151-158.

DOI：10.1016/1050-3862(91)90024-L

Genomic mapping studies frequently employ retrovirus-mediated transfer of dominant selectable markers to specific target chromosomes. DNA probes containing sequences adjacent to inserted proviruses are valuable mapping tools in such studies. We have implemented a strategy for amplification of chromosomal sequences flanking the 5' LTR of MoMuLV-based vectors. Probes derived from these amplification products successfully differentiated murine versus human proviral localization in retrovirus-infected mouse-human chromosome 17q hybrid cells.

• 出版日期1991-8