A comparison of the inclusion behavior of human serum albumin and holo transferrin with fluoxymesterone in the presence of three different cyclodextrins

作者：Ghaderabad Maryam; Mansouri Mahdieh; Beigoli Sima; Rad Atena Sharifi; Mehrzad Jamshid; Saberi Mohammad Reza; Chamani Jamshidkhan^*

来源：Journal of the Iranian Chemical Society, 2017, 14(7): 1347-1364.

DOI：10.1007/s13738-017-1085-1

• Human serum albumin
• Human serum transferrin
• Cyclodextrins (alpha, beta and gamma)
• Fluorescence quenching
• Molecular modeling

This article describes the interaction of fluoxymesterone (Flu) with HSA and HTF in the absence and presence of cyclodextrins (CDs) (alpha, beta and gamma). According to fluorescence data, the binding of Flu to the proteins caused strong static quenching in the binary and ternary systems. The fluorescence quenching results demonstrated that HSA and HTF had two and one class of apparent binding sites with a distinct binding constant in the presence of the CDs, respectively. The effects of Flu on the structure of HSA and HTF were analyzed using synchronous fluorescence spectroscopy, which showed that the interaction of Flu with both proteins in the binary and ternary systems altered the microenvironment around the Trp and Tyr residues. The distance, r, between Flu and the proteins was obtained according to FRET which pointed at a successful formation of a drug-protein complex. Far-UV CD spectra indicated that the binding of the drug to both proteins induced changes in the secondary structure of HSA and HTF in the binary and ternary systems. Finally, molecular modeling provided possible binding sites of Flu within the proteins for the binary and ternary systems and also confirmed the experimental results. The obtained data can be useful for determining usage drug doses in drug delivery.

• 出版日期2017-7