Maternal pregravid obesity changes gene expression profiles toward greater inflammation and reduced insulin sensitivity in umbilical cord

作者：Thakali Keshari M; Saben Jessica; Faske Jennifer B; Lindsey Forrest; Gomez Acevedo Horacio; Lowery Curtis L Jr; Badger Thomas M; Andres Aline; Shankar Kartik^*

来源：Pediatric Research, 2014, 76(2): 202-210.

DOI：10.1038/pr.2014.72

BACKGROUND: Maternal obesity is associated with unfavorable outcomes, which may be reflected in the as yet undiscovered gene expression profiles of the umbilical cord (UC). %26lt;br%26gt;METHODS: UCs from 12 lean (pregravid BMI %26lt; 24.9) and 10 overweight/obese (pregravid BMI %26gt;= 25) women without gestational diabetes were collected for gene expression analysis using Human Primeview microarrays. Metabolic parameters were assayed in mother%26apos;s plasma and cord blood. %26lt;br%26gt;RESULTS: Although offspring birth weight and adiposity (at 2 wk) did not differ between groups, expression of 232 transcripts was affected in UC from overweight/obese compared with those of lean mothers. Gene-set enrichment analysis revealed an upregulation of genes related to metabolism, stimulus and defense response, and inhibitory to insulin signaling in the overweight/obese group. We confirmed that EGR1, periostin, and FOSB mRNA expression was induced in UCs from overweight/obese mothers, while endothelin receptor B, KLF10, PEG3, and EGLN3 expression was decreased. Messenger RNA expression of EGR1, FOSB, MEST, and SOCS1 were positively correlated (P %26lt; 0.05) with mother%26apos;s first-trimester body fat mass (%). %26lt;br%26gt;CONCLUSION: Our data suggest a positive association between maternal obesity and changes in UC gene expression profiles favoring inflammation and insulin resistance, potentially predisposing infants to develop metabolic dysfunction later on in life.

• 出版日期2014-8