A DNA-templated silver nanocluster probe for label-free, turn-on fluorescence-based screening of homo-adenine binding molecules

作者：Park Ki Soo; Park Hyun Gyu^*

来源：Biosensors and Bioelectronics, 2015, 64: 618-624.

DOI：10.1016/j.bios.2014.10.003

• Fluorescent biosensors
• DNA-templated silver nanoclusters
• Fluorescence signal enhancement
• Homo-adenine binding molecules
• Coralyne

A novel, label-free, turn-on fluorescence strategy to detect molecules that bind to adenine-rich DNA sequences has been developed. The probe employs DNA-templated silver nanoclusters (DNA-AgNCs) as the key detection component. The new strategy relies on the formation of non-Watson-Crick homo-adenine DNA duplex, triggered by strong interactions with homo-adenine binding molecules, which brings a guanine-rich sequence in one strand close to DNA-AgNCs located on the opposite strand. This phenomenon transforms weakly fluorescent AgNCs into highly emissive species that display bright red fluorescence. Finally, we have shown that the new fluorescence turn-on strategy can be employed to detect coralyne, the most representative homo-adenine binding molecule that triggers formation of a non-Watson-Crick homo-adenine DNA duplex.

• 出版日期2015-2-15