Unique roles of Schistosoma japonicum protein Sj16 to induce IFN-γ and IL-10 producing CD4+CD25+ regulatory T cells in vitro and in vivo

作者：Sun, X. J.; Li, R.; Sun, X.; Zhou, Y.; Wang, Y.^*; Liu, X. J.; Lu, Q.; Zhou, C. L.; Wu, Z. D.

来源：Parasite Immunology, 2012, 34(8-9): 430-439.

DOI：10.1111/j.1365-3024.2012.01377.x

• BMDCs
• CD4+CD25+regulatory T cells
• IFN-
• Schistosoma japonicum
• Sj16

Various proteins are expressed during different stages of schistosome development that are essential for cercarial penetration of vertebrate skin and evasion of host immune response. CD4+CD25+ regulatory T cells are important in modulating immune responses towards helminth infections. Schistosoma japonicum protein Sj16 present in the secretions of schistosomula has been shown to have anti-inflammatory effects; however, it is uncertain whether Sj16 can induce CD4+CD25+ regulatory T cells to participate in the regulation of early infection. In this study, we demonstrate a relationship between recombinant Sj16 (rSj16) and the induction of CD4+CD25+ Foxp3+ regulatory T cells. An increase in CD4+CD25+ T cells was observed both in splenic cells from mice injected with rSj16 and the cells pretreated with rSj16, respectively. The induced CD4+CD25+ T cells suppressed CD4+CD25- T-cell proliferation; furthermore, IFN-? and IL-10 released from rSj16-stimulated cells contribute to this suppression. Additionally, rSj16-treated bone marrow dendritic cells (BMDCs) demonstrate an immature phenotype and play a role in the conversion of CD4+CD25- T cells into suppressive CD4+CD25+ regulatory T cells. Our study identified a new CD4+CD25+ T-cell population that induced by rSj16 and suggests that an IFN-?-biased microenvironment during early infection of schistosome may favour the establishment of infection.

• 出版日期2012-8
• 单位南京医科大学; 中山大学