An efficient and simple method for site-selective modification of O-6-methyl-2 %26apos;-deoxyguanosine in DNA

作者：Onizuka Kazumitsu; Nishioka Takamasa; Li Zhichun; Jitsuzaki Daichi; Taniguchi Yosuke; Sasaki Shigeki^*

来源：Chemical Communications, 2012, 48(33): 3969-3971.

DOI：10.1039/c2cc17621a

O-6-Methyl-20-deoxyguanosine (O-6-Me-dG) is a mutagenic nucleotide in DNA. O-6-Me-dG in DNA was rapidly and selectively modified by a functionality transfer reaction using the ODN incorporating 6-S-functionalized thioguanosine. Subsequent labelling of O-6-Me-dG with the fluorescent FAM or biotin group via click chemistry has permitted the sensitive and selective detection of O-6-Me-dG in DNA.

• 出版日期2012