Size reversion of a truncated DNA beta associated with Tobacco curly shoot virus

作者：Qian Yajuan; Tan Zhong; Liu Yi; Briddon Rob W; Zhou Xueping^*

来源：Virus Research, 2008, 131(2): 288-292.

DOI：10.1016/j.virusres.2007.09.005

• Tobacco curly shoot virus
• beta C1 gene
• DNA beta
• size reversion
• recombinant
• T-DNA

Our previous results demonstrated that DNA beta associated with Tobacco curly shoot virus (TbCSV) is not necessary for infection but intensifies symptoms in some hosts. To better understand the function of DNA beta in virus infection, a beta C1 deleted infectious clone of the TbCSV DNA beta was constructed. Agroinoculation showed that the truncated DNA beta (DNA Delta C1 beta) was trans-replicated by TbCSV in tobacco and Petunia hybrida plants. However, PCR and Southern blot analysis demonstrated that the truncated DNA beta reverted to near wild type component size in some Nicotiana benthamiana, N. glutinosa, N. tabacum Samsun and P. hybrida plants co-inoculated with TbCSV and DNA Delta C1 beta. Sequence analysis of four DNA Delta C1 beta derivatives revealed that the wild type size DNA beta molecules were recombinants between TbCSV DNA beta and the pBinPLUS vector in which dimeric constructs were produced for inoculation. The significance of these findings is discussed with respect to the constraints imposed on begomovirus genome size.

• 出版日期2008-2
• 单位浙江大学