A simple strategy for the separation and purification of water-soluble polysaccharides from the fresh Spirulina platensis

作者:Wang, Feng*; Ma, Yong; Liu, Yanhua; Cui, Zhenggang; Ying, Xiaoyan; Zhang, Fuming; Linhardt, Robert J.
来源:Separation Science and Technology (Philadelphia), 2017, 52(3): 456-466.
DOI:10.1080/01496395.2016.1244549

摘要

A novel and industrially applicable strategy for separating and purifying the water-soluble polysaccharides from the fresh Spirulina platensis is introduced in this study. After hot water extraction, using chitosan flocculation treatment, 86.0% of the particles and 85.9% of the pigments in the crude extract were removed. The purity of polysaccharides could be increased to 94.6% and 96.1% by applying the macroporous resin ADS-7 to remove the impurities in the mode of static adsorption and in a dynamic mode of operation, respectively. The polysaccharides obtained were composed of three major components having molecular weights of 1400, 420 and 2 kDa.