Allelic polymorphism, mRNA and antigen expression of KIR2DL1 in the Chinese Han population

作者：He Yanmin; Tao Sudan; Ying Yanling; He Ji; Zhu Faming^*; Lv Hangjun

来源：Human Immunology, 2014, 75(3): 245-249.

DOI：10.1016/j.humimm.2013.12.005

KIR2DL1 is one important molecule of inhibitory receptors that recognizes a subset of HLA-C allelic products carrying Lys80. In this study, we have investigated the allelic polymorphism, mRNA and antigen expression level of KIR2DL1 in the Chinese Han population. KIR2DL1*001,*00302 and *00401 alleles and seven genotypes including two copy 2DL1*00302, one copy 2DL1*00302, two copy 2DL1*001, one copy 2DL1*001, 2DL1*00302/2DL1*001, 2DL1*001/2DL1*00401, 2DL1*00302/2DL1*00401 were identified in the total 164 Chinese Han individuals. The frequency of NK cells expression KIR2DL1 was varied considerably. There was no disparity on the level of antibody-binding for different genotypes according to mean fluorescent intensity and there was almost similar frequency of antibody-binding NK cells except for group KIR2DL1*00302 with one copy. The frequency of NK cells expression KIR2DL1 of the individuals in the group 2DL1*00302 with one copy was lower than that in the group 2DL1*00302 with two copies. The amount of transcript was variable among the individuals and the mean value of transcript abundance in 21 individuals with one copy was lower than that in 143 individuals with two copies. In conclusion, the frequency of NK cells expression KIR2DL1 and the mRNA transcript abundance were not associated with allelic polymorphism but with gene copy number.

• 出版日期2014-3
• 单位浙江省血液中心