An efficient plant regeneration system with in vitro flavonoid accumulation for Hylotelephium tatarinowii (Maxim.) H. Ohba

作者：Wang Junli^*; Wang Jue; Liu Kun; Xiao Xuan; Gong Weizhen; Lu Yuan; Liu Mingfei; Xu Dongting

来源：In Vitro Cellular and Developmental Biology - Plant, 2010, 46(5): 445-450.

DOI：10.1007/s11627-010-9315-6

• Hylotelephium tatarinowii
• Regeneration
• Flavonoids
• Germplasm conservation

An efficient micropropagation system for Hylotelephium tatarinowii (Maxim.) H. Ohba, a rare medicinal plant, has been developed. Callus induced from leaf explants placed onto Murashige and Skoog (MS) medium with supplementation of plant growth regulators. When the concentration of 2,4-dicholorophenoxy acetic acid was as high as 2.0 mg l(-1) in combination with 0.5 mg l(-1) 6-benzylaminopurine (6-BAP), the callus induction rate reached 92.1%. Adventitious shoots were observed on callus exposed to 1.0 mg l(-1) 6-BAP, with 81.5% frequency of shoot regeneration after 30 d. Flower buds appeared after subculture. Regenerated shoots could flower normally in vitro. Up to 100% of the regenerated shoots formed complete plantlets on half-strength MS medium without any growth regulator, with an average of 5.9 roots per shoot explant. Quantitative analysis of flavonoids and rutin showed that the phytochemical profile of callus and regenerated plants was similar to that of wild plants.

• 出版日期2010-10
• 单位中央民族大学