Neolignans from the Fruits of Magnolia obovata and Their Inhibition Effect on NO Production in LPS-Induced RAW264.7 Cells

作者：Seo Kyeong Hwa; Lee Dae Young; Lee Dong Sung; Park Ji Hae; Jeong Rak Hun; Jung Ye Jin; Shrestha Sabina; Chung In Sik; Kim Geum Soog; Kim Youn Chul; Baek Nam In^*

来源：Planta Medica, 2013, 79(14): 1335-1340.

DOI：10.1055/s-0033-1350673

• anti-inflammation
• Magnoliaceae
• Magnolia obovata
• magnobovatol
• 9-methoxyobovatol
• neolignan
• NO production

Three new neolignans, named 9-methoxyobovatol (6), magnobovatol (7), and 2-hydroxyobovaaldehyde (9), along with six known ones, magnolol (1), honokiol (2), isomagnolol (3), obovatol (4), obovatal (5), and obovaaldehyde (8), were isolated from the fruits of Magnolia obovata using silica gel and ODS column chromatography. From the results of spectroscopic data including EIMS, IR, H-1- and C-13-NMR, DEPT, and 2D-NMR (gCOSY, gHSQC, gHMBC), the chemical structures were determined. All isolated compounds were evaluated for inhibition activity on nitric oxide production in LPS-induced RAW 264.7 cells, and compounds 1-4, 6, 7, and 9 showed significant activity with IC50 values of 15.8 +/- 0.3, 3.3 +/- 1.2, 14.1 +/- 0.9, 6.2 +/- 1.2, 14.8 +/- 2.3, 14.2 +/- 1.2, and 14.8 +/- 3.2 mu M, respectively, without any visible toxic effect.

• 出版日期2013-9