Anti-inflammatory effect of natural and semi-synthetic phthalides

作者：Del Angel Mayela; Nieto Antonio; Ramirez Apan Teresa; Delgado Guillermo^*

来源：European Journal of Pharmacology, 2015, 752: 40-48.

DOI：10.1016/j.ejphar.2015.01.026

• Inflammation
• Semi-synthetic phthalides
• 12-O-tetradecanoylphorbol-13-acetate
• Carrageenan
• Myeloperoxidase
• Oxide nitric synthase

This study evaluated the potential anti-inflammatory effects of natural phthalides, isolated from Ligusticum porteri, and of semi-synthetic phthalides. Anti-inflammatory activity was investigated in two mouse models; one with ear edema, induced with 12-O-tetradecanoylphorbol-13-acetate, and the other with paw edema, induced with carrageenan. The effect on the RAW 264.7 stimulated with lipopolysaccharicle cells was evaluated and after application of 12-O-tetradecanoylphorbol-13-acetate, the activity of myeloperoxiclase was assessed to serve as an index of leukocytes infiltration together with the histological evaluations. We also assessed the inhibition of cyclooxygenases 1 and 2 in vitro. Our results demonstrated that administration of semi-synthetic phthalides significantly inhibited the ear edema induced by 12-O-tetradecanoylphorbol-13-acetate, and reduced the paw edema caused by carrageenan. The anti-inflammatory activity of phthalides could, in part, be explained by the reduction in myeloperoxiclase activity and the infiltration of leukocytes. The semi-synthetic phthalicles also inhibited the production of oxide nitric in RAW cells.

• 出版日期2015-4-5