Neuronal pannexin-1 channels are not molecular routes of water influx during spreading depolarization-induced dendritic beading

作者：Sword Jeremy; Croom Deborah; Wang Phil L; Thompson Roger J; Kirov Sergei A^*

来源：Journal of Cerebral Blood Flow and Metabolism, 2017, 37(5): 1626-1633.

DOI：10.1177/0271678X16639328

• Conditional pannexin-1 knock-out
• mefloquine
• neuronal swelling
• two-photon imaging
• viral vectors

Spreading depolarization-induced focal dendritic swelling (beading) is an early hallmark of neuronal cytotoxic edema. Pyramidal neurons lack membrane-bound aquaporins posing a question of how water enters neurons during spreading depolarization. Recently, we have identified chloride-coupled transport mechanisms that can, at least in part, participate in dendritic beading. Yet transporter-mediated ion and water fluxes could be paralleled by water entry through additional pathways such as large-pore pannexin-1 channels opened by spreading depolarization. Using real-time in vivo two-photon imaging in mice with pharmacological inhibition or conditional genetic deletion of pannexin-1, we showed that pannexin-1 channels are not required for spreading depolarization-induced focal dendritic swelling.

• 出版日期2017-5