科研之友
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摘要
Delivering stem/progenitor cells via a degradable synthetic membrane to devitalized allogenic tissue graft surfaces presents a promising allograftmediated tissue regeneration strategy. However, balancing degradability and bioactivity of the synthetic membrane with physical characteristics demanded for successful clinical translation is challenging. Here, well-integrated composites of hydroxyapatite (HA) and amphiphilic poly(lactide-co-glycolide)b- poly(ethylene glycol)-b-poly(lactide-co-glycolide) (PELGA) with tunable degradation rates are designed that stiffen upon hydration and exhibit excellent shape recovery ability at body temperature for efficiently delivering skeletal progenitor cells around bone grafts. Unlike conventional degradable polymers that weaken upon wetting, these amphiphilic composites stiffen upon hydration as a result of enhanced polyethylene glycol (PEG) crystallization. HA-PELGA composite membranes support the attachment, proliferation, and osteogenesis of rat periosteum-derived cells in vitro, as well as the facile transfer of confluent cell sheets of green fluorescent protein-labeled bone marrow stromal cells. With efficient shape memory behaviors around physiological temperature, the composite membranes can be programmed with a permanent tubular configuration, deformed into a flat temporary shape desired for cell seeding/cell sheet transfer, and triggered to wrap around a femoral bone allograft upon 37 degrees C saline rinse and subsequently stiffen. These properties combined make electrospun HA-PELGA promising smart synthetic periosteal membranes for augmenting allograft healing.
- 出版日期2017-2
