Analysis of Cry8Ka5-binding proteins from Anthonomus grandis (Coleoptera: Curculionidae) midgut

作者：Nakasu Erich Y T; Firmino Alexandre A P; Dias Simoni C; Rocha Thales L; Ramos Hudson B; Oliveira Gustavo R; Lucena Wagner; Carlini Celia R; Grossi de Sa Maria Fatima^*

来源：Journal of Invertebrate Pathology, 2010, 104(3): 227-230.

DOI：10.1016/j.jip.2010.01.012

• Anthonomus grandis
• Bacillus thuringiensis
• Cry8Ka5
• Two-dimensional gel electrophoresis

Biotech crops expressing Bacillus thuringiensis Cry toxins present a valuable approach for insect control. Cry8Ka5, which is highly toxic to the cotton boll weevil (Anthonomus grandis), was used as a model to study toxin-ligand interactions. Three Cry-binding proteins were detected after toxin overlay assays. Following de novo sequencing, a heat-shock cognate protein and a V-ATPase were identified, whilst a similar to 120 kDa protein remained unknown. Additional Cry8Ka5-binding proteins were visualized by two-dimensional gel electrophoresis ligand blots.

• 出版日期2010-7