A perspective on conformational control of electron transfer in nitric oxide synthases

作者：Hedison Tobias M; Hay Sam; Scrutton Nigel S^*

来源：Nitric Oxide-Biology and Chemistry, 2017, 63: 61-67.

DOI：10.1016/j.niox.2016.09.002

• Nitric oxide synthase
• Cytochrome P450 reductase
• Diflavin oxidoreductase
• Fluorescence
• Protein dynamics

This perspective reviews single molecule and ensemble fluorescence spectroscopy studies of the three tissue specific nitric oxide synthase (NOS) isoenzymes and the related diflavin oxidoreductase cyto chrome P450 reductase. The focus is on the role of protein dynamics and the protein conformational landscape and we discuss how recent fluorescence-based studies have helped in illustrating how the nature of the NOS conformational landscape relates to enzyme turnover and catalysis.

• 出版日期2017-2-28