A new method for hydroxyl radical detection by chemiluminescence of flue-cured tobacco extracts

作者：Wang, Dabin; Yu, Weisong; Pang, Xueli; Cao, Jianmin; Qiu, Jun^*; Kong, Fanyu^*

来源：Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy , 2018, 204: 436-439.

DOI：10.1016/j.saa.2018.06.051

• Chemiluminescence
• pH
• Tobacco-methanol extract
• Hydroxyl radical detection

Chemiluminescence (CL) reactions usually take place in a severely restricted pH regime, thereby confining their application in media at different pH. In this paper, the CL behavior of tobacco-methanol extract (TME) was explored. Surprisingly, TME exhibited CL behavior upon mixing with Fe2+/H2O2, HRP/H2O2 and gold nanoparticles/H2O2 oxidation systems, i.e., in acidic, neutral and alkaline solution respectively. Addition of different reactive oxygen species scavengers verified that the hydroxyl radical (center dot OH) triggers TME CL reactions. Then, the CL behavior of TME was applied to determine center dot OH in tetrachloro-1,4-benzoquinone (TCBQ)/H2O2 system in acidic, neutral and alkaline solutions. CL intensity correlated most strongly (R-2 = 0.99) with TCBQ concentration, which was used as a means to indirectly denote center dot OH concentration. This implies that center dot OH could be determined by a TME CL method in a semi-quantitative way regardless of pH value. Therefore, the TME CL method may be a promising method for center dot OH determination in various center dot OH-generating systems.

• 出版日期2018-11-5
• 单位中国农业科学院