Automated particle picking for low-contrast macromolecules in cryo-electron microscopy

作者：Langlois Robert; Pallesen Jesper; Ash Jordan T; Ho Danny Nam; Rubinstein John L; Frank Joachim^*

来源：Journal of Structural Biology, 2014, 186(1): 1-7.

DOI：10.1016/j.jsb.2014.03.001

• Particle selection
• Machine-learning
• Automation
• High-resolution
• Cryo-EM

Cryo-electron microscopy is an increasingly popular tool for studying the structure and dynamics of biological macromolecules at high resolution. A crucial step in automating single-particle reconstruction of a biological sample is the selection of particle images from a micrograph. We present a novel algorithm for selecting particle images in low-contrast conditions; it proves more effective than the human eye on close-to-focus micrographs, yielding improved or comparable resolution in reconstructions of two macromolecular complexes.

• 出版日期2014-4