Embryotoxicity of cobalt ferrite and gold nanoparticles: A first in vitro approach

作者：Di Guglielmo Claudia; Ramos Lopez David; De Lapuente Joaquin; Llobet Mallafre Joan Maria; Borras Suarez Miguel

来源：Reproductive Toxicology, 2010, 30(2): 271-276.

DOI：10.1016/j.reprotox.2010.05.001

• Mouse embryonic stem cells test
• Teratology
• Embryotoxicity
• Gold nanoparticles
• Cobalt ferrite nanoparticles
• Cytotoxicity
• Differentiation

Nanoparticles (NPs) are emerging as promising biomedical tools thanks to their peculiar characteristics. Our purpose was to investigate the embryotoxicity of cobalt ferrite and gold NPs through the Embryonic Stem Cell Test (EST). The EST is an in vitro standard assay, which permits to classify substances as strongly, weakly or non-embryotoxic. Due to the particular physical-chemical nature of nanoparticles, we introduced a modification to the standard protocol exposing the Embryonic Stem Cells (ES-D3) to nanoparticles only during the first 5 days of the assay. Moreover, we proposed a method to discriminate and compare the embryotoxicity of the substances within the weakly embryotoxic range. Our ID(50) results permit to classify cobalt ferrite nanoparticles coated with gold and silanes as non-embryotoxic. The remaining nanoparticles have been classified as weakly embryotoxic in this decreasing order: gold salt (HAuCl(4)center dot 3H(2)O) > cobalt ferrite salt (CoFe(2)O(4)) > cobalt ferrite nanoparticles coated with silanes (Si-CoFe) > gold nanoparticles coated with hyaluronic acid (HA-Au).

• 出版日期2010-9