Direct and Rapid Quantification of Calcium Phosphate Precipitate on Titanium by X-Ray Fluorescence Analysis Using Fundamental Parameter Method

作者：Satoh Kozue^*; Komata Masashi; Mizuhira Manabu; Wagatsuma Kazuaki; Ohtsu Naofumi

来源：Journal of the Japan Institute of Metals, 2015, 79(1): 1-8.

DOI：10.2320/jinstmet.J2014026

• X-ray fluorescence analysis
• fundamental parameter method
• thin film
• calcium phosphate
• titanium
• metallic biomaterial
• bioactivity

In the present study, we propose a characterization technique to determine the amount of calcium phosphate (CP) precipitate formed on a titanium substrate. The quantitative analysis of the CP precipitate on a metallic substrate is significant for researchers of metallic biomaterials because CP that spontaneously precipitates in a simulated-body fluid gives information on the bioactivity of the metallic biomaterials. We focused on X-ray fluorescence (XRF) analysis and adopted the thin-film fundamental parameter method (thin-film FP method) because it allows direct (non-pretreatment) and rapid quantitative analysis without any reference materials. We show that XRF analysis using the thin-film FP method can be adequately applied to the quantitative analysis of the CP precipitate in the simulated-body fluid immersion test. We also show that the density of the CP-precipitate layer can be estimated by combining the XRF results with images of cross-sectional scanning electron microscopy (SEM).
Consequently, XRF analysis combined with the thin-film FP method can provide a convenient means to evaluate the CP precipitate on a titanium substrate, which improves the accuracy and accessibility of the simulated-body fluid immersion test.

• 出版日期2015-1