Direct visual detection of DNA based on the light scattering of silica nanoparticles on a human papillomavirus DNA chip

作者：Piao Jing Yu; Park Eun Hee; Choi Kihwan; Quan Bo; Kang Dong Ho; Park Pan Yun; Kim Dai Sik; Chung Doo Soo^*

来源：Talanta, 2009, 80(2): 967-973.

DOI：10.1016/j.talanta.2009.08.024

• Plain silica nanoparticle
• Light scattering
• Aggregation
• Visual detection

A detection system for a human papillomavirus (HPV) DNA chip based on the light scattering of aggregated silica nanoparticle probes is presented. In the assay, a target HPV DNA is sandwiched between the capture DNA immobilized on the chip and the probe DNA immobilized on the plain silica nanoparticle. The spot where the sandwich reaction occurs appears bright white and is readily distinguishable to the naked eye. Scanning electron microscopy images clearly show the aggregation of the silica nanoparticle probes. When three different sized (55 nm, 137 rim, 286 nm) plain silica nanoparticles were compared, probes of the larger silica nanoparticles showed a higher scattering intensity. Using 286-nm silica nanoparticles, the spots obtained with 200 pM of target DNA were visually detectable. The demonstrated capability to detect a disease related target DNA with direct visualization without using a complex detection instrument provides the prerequisite for the development of portable testing kits for genotyping.

• 出版日期2009-12-15