Accumulation of FoxP3-expressing CD4( )CD25( ) T cells with distinct chemokine receptors in synovial fluid of patients with active rheumatoid arthritis

作者：Jiao, Z^*; Wang, W; Jia, R; Li, J; You, H; Chen, L; Wang, Y

来源：Scandinavian Journal of Rheumatology, 2007, 36(6): 428-433.

DOI：10.1080/03009740701482800

Objective: To explore the presence and characteristics of FoxP3-expressing CD4( )CD25( ) regulatory T cells in synovial fluid (SF) of patients with active rheumatoid arthritis (RA).
Methods: The frequency and chemokine receptors expression profile of FoxP3-expressing CD4( )CD25( ) regulatory T cells in SF and peripheral blood (PB) from RA patients and PB from healthy controls were investigated by flow cytometry using three- or four-colour intracellular staining.
Results: The frequency of CD4( )CD25( ) FoxP3( ) T cells was increased significantly in SF compared with paired PB from RA patients and PB from healthy controls (p < 0.05). However, the frequency in PB from RA patients was significantly lower than in PB from healthy controls (p < 0.05). Notably, CD4( )CD25( )FoxP3( ) T cells in SF expressed increased levels of inflammation-related trafficking chemokine receptors, such as CCR4, CCR5, and CXCR4.
Conclusion: There is an accumulation of FoxP3-expressing regulatory T cells in RA SF, and such recruitment may be dependent on the distinct chemokine receptors expressed on regulatory T cells.

• 出版日期2007-12
• 单位上海交通大学; 江苏大学