A comparative study of dietary curcumin, nanocurcumin, and other classical amyloid-binding dyes for labeling and imaging of amyloid plaques in brain tissue of 5x-familial Alzheimer%26apos;s disease mice

作者：Maiti Panchanan; Hall Tia C; Paladugu Leela; Kolli Nivya; Learman Cameron; Rossignol Julien; Dunbar Gary L

来源：Histochemistry and Cell Biology, 2016, 146(5): 609-625.

DOI：10.1007/s00418-016-1464-1

• Curcumin
• Nanocurcumin
• Protein labelling
• Alzheimer's disease
• Amyloid beta protein

Deposition of amyloid beta protein (A beta) is a key component in the pathogenesis of Alzheimer's disease (AD). As an anti-amyloid natural polyphenol, curcumin (Cur) has been used as a therapy for AD. Its fluorescent activity, preferential binding to A beta, as well as structural similarities with other traditional amyloid-binding dyes, make it a promising candidate for labeling and imaging of A beta plaques in vivo. The present study was designed to test whether dietary Cur and nanocurcumin (NC) provide more sensitivity for labeling and imaging of A beta plaques in brain tissues from the 5x-familial AD (5xFAD) mice than the classical A beta-binding dyes, such as Congo red and Thioflavin-S. These comparisons were made in postmortem brain tissues from the 5xFAD mice. We observed that Cur and NC labeled A beta plaques to the same degree as A beta-specific antibody and to a greater extent than those of the classical amyloid-binding dyes. Cur and NC also labeled A beta plaques in 5xFAD brain tissues when injected intraperitoneally. Nanomolar concentrations of Cur or NC are sufficient for labeling and imaging of A beta plaques in 5xFAD brain tissue. Cur and NC also labeled different types of A beta plaques, including core, neuritic, diffuse, and burned-out, to a greater degree than other amyloid-binding dyes. Therefore, Cur and or NC can be used as an alternative to A beta-specific antibody for labeling and imaging of A beta plaques ex vivo and in vivo. It can provide an easy and inexpensive means of detecting A beta-plaque load in postmortem brain tissue of animal models of AD after anti-amyloid therapy.

• 出版日期2016-11