A Novel Polysaccharide Compound Derived from Algae Extract Stimulates Insulin Secretion of Pancreatic beta MIN-6 Cells

作者：Xie Peiyu; Fujii Isao; Zhao Ji' en; Shinohara Makoto; Matsukura Makoto^*

来源：International Medical Journal, 2012, 19(3): 193-197.

• polysaccharide compound
• algae extract
• pancreatic beta MIN-6 cells
• insulin secretion
• anti-diabetic

Background: Interest in biological activities of compound derived from algae extracts has been intensified. %26lt;br%26gt;Objective: Investigate the stimulatory activity of a novel polysaccharide compound derived from algae extract on insulin secretion of mice pancreatic beta MIN-6 cells. %26lt;br%26gt;Design: We prepared glybenclamide, a commonly prescribed sulfonylurea (SU) against diabetes mellitus type 2 (non-insulin-dependent diabetes mellitus), and a novel polysaccharide compound derived from algae extract to investigate their stimulatory activity. Amylin (IAPP; islet amyloid polypeptide), an inhibitor for glybenclamide, was used to elucidate the different mechanisms between glybenclamide and the polysaccharide compound. %26lt;br%26gt;Methods: Insulin enzyme immunoassay (EIA) system was used to determine insulin secretion of pancreatic beta MIN-6 cells induced by glybenclamide and the polysaccharide compound in a dose-dependent manner and a time-dependent manner, and amylin was used as an inhibitor. Cell Titer 96 (R) AQ(ueous) one solution cell proliferation assay kit was used to analyze the cytotoxicity of the polysaccharide compound. %26lt;br%26gt;Results: Both glybenclamide and the polysaccharide compound could stimulate the insulin secretion of pancreatic beta MIN-6 cells. The polysaccharide compound did not show apparent cytotoxicity in a dose-dependent manner, in contrast, it seemed that glybenclamide was more toxic than the polysaccharide compound. Amylin was an inhibitor for glybenclamide, but didn%26apos;t inhibit the activity of the polysaccharide compound. %26lt;br%26gt;Conclusion: The polysaccharide compound could stimulate the insulin secretion of pancreatic beta MIN-6 cells through a different mechanism from glybenclamide without apparent cytotoxicity. The results provided a basis for the development of living marine resources.

• 出版日期2012-9