Use of the V(H)6-1 gene segment to code for anti-interleukin-18 autoantibodies in multiple sclerosis

作者：Tiumentseva Marina^*; Morozova Vera; Zakabunin Aleksandr; Korobko Denis; Malkova Nadezhda; Filipenko Maksim; Tikunova Nina

来源：Immunogenetics, 2016, 68(4): 237-246.

DOI：10.1007/s00251-015-0895-5

• Immunoglobulin genes
• Autoantibodies
• Interleukin (IL)-18
• Multiple sclerosis
• Phage display

We investigated whether levels and repertoires of anti-interleukin-18 (IL-18) autoantibodies (auto-Abs) differ in multiple sclerosis (MS) patients and healthy donors (HDs). IL-18 concentration in MS patients' sera was higher than in HD, but the level of anti-IL-18 auto-Abs was lower in MS patients. Correlation patterns of IL-18/anti-IL-18 auto-Abs system differed in HD and MS patients, so we have compared segment composition of the anti-IL-18 single-chain variable fragments (scFvs) selected from MS and na < ve phage display libraries. Considerable differences between anti-IL-18 auto-Abs of these libraries were found. MS panel contained auto-Abs displaying both signs of "fetal" and somatically hypermutated repertoires. Na < ve panel mainly contained the na < ve antibodies. These variations from the norm are possible results of abnormal regulation of the repertoire perhaps determined by remodeling of the molecular mechanisms involved in the V(D)J recombination and/or abnormal selection by antigen in MS pathogenesis.

• 出版日期2016-4