GREEN SYNTHESIS OF SILVER NANOPARTICLES BY USING STEM DERIVED CALLUS EXTRACT OF BITTER APPLE (CITRULLUS COLOCYNTHIS)

作者：Satyavani K^*; Ramanathan T; Gurudeeban S

来源：Digest Journal of Nanomaterials and Biostructures, 2011, 6(3): 1019-1024.

• Atomic Force Microscopy
• Callus
• Citrullus colocynthis
• Polyphenols
• Silver Nanoparticles

Plant mediated synthesis of metallic nanoparticles is an increasing commercial demand due to the wide applicability in various areas such as electronics, catalysis, chemistry, energy, cosmetics and medicine. In the present investigation, synthesis of silver nanoparticles by using stem derived callus extracts of Citrullus colocynthis (L.) Schrader. The extract incubated with AgNO(3) showed gradual change in the colour of the extract from greenish to reddish brown it indicate the silver nanopaticles synthesis. The shape of the SNP synthesized by stem derived callus extract was spherical and was found to be in the range of 75 nm by AFM. FTIR absorption spectra conclude that the compounds attached with silver nanoparticles could be polyphenols with aromatic ring and bound amide region. The novel silver nanoparticles exhibited a tremendous antibacterial activity; it showed the maximum activity against bioflim bacteria such as E. coli (10.1 mm), V. paraheamolyticus (10.1 mm), P. aeruginosa (8 mm), Proteus vulgaris (9 mm) and L. monocytogens (8 mm) and also observed that it showed no activity against Proteus mirabilis, Salmonella enteritidis, and Staphylococcus aureus. To the best of our knowledge this is the first report on the antibacterial activity of silver nanoparticles against bioflim forming bacteria.

• 出版日期2011-9