An extracellular endo-1,4-beta-xylanase from Aspergillus japonicus: Purification, properties, and characterization of the encoding gene

作者：Wakiyama Motoki; Yoshihara Koji; Hayashi Sachio; Ohta Kazuyoshi^*

来源：Journal of Bioscience and Bioengineering, 2010, 109(3): 227-229.

DOI：10.1016/j.jbiosc.2009.09.005

• Aspergillus japonicas
• Endo-1,4-beta-xylanase
• Extracellular enzyme
• Glycoside hydrolase family 11
• Xylan

An extracellular endo-1,4-beta-xylanase with specific activity of 566 U/mg was purified from the culture filtrate of a filamentous fungus, Aspergillus japonicus strain MU-2, grown on oat spelt xylan. The purified enzyme showed a single band on SDS-PAGE with an apparent M(r) of 25.1 kDa. Xylanase activity was optimal at pH 5.0 and 60 degrees C. The xylanase gene (xynA) encoded a 42 residue prepropeptide and a 191 residue mature protein. The XynA protein showed the highest sequence identity of 69% to Aspergillus niger XynB (DQ174549), which belongs to the glycoside hydrolase family 11.

• 出版日期2010-3