An abundant acyl-CoA (Delta 9) desaturase transcript in pheromone glands of the cabbage moth, Mamestra brassicae, encodes a catalytically inactive protein

作者：Park Hee Yun; Kim Myung Sook; Paek Aron; Jeong Seong Eun^*; Knipple Douglas C

来源：Insect Biochemistry and Molecular Biology, 2008, 38(5): 581-595.

DOI：10.1016/j.ibmb.2008.02.001

• pheromone gland
• acyl-CoA desaturase
• functional expression
• missense mutation
• Lepidoptera
• Noctuidae
• Hadeninae
• Mamestra brassicae

The principal sex pheromone component produced by females of the cabbage moth, Mamestra brassicae, is derived from the monounsaturated fatty acid, Z11-16:1, whereas two additional trace components are derived from E11-16:1 and Z9-16:1. This report presents the isolation and analysis of cDNAs encoding pheromone gland-specific acyl-CoA desaturases implicated in the production of these unsaturated fatty acids (UFAs). Comparisons of the encoded amino acid sequences of four cDNA fragments isolated by degenerate PCR from cabbage moth pheromone glands established their orthology with previously characterized noctuid desaturases as follows: MbraLPAQ, belonging to the pheromone gland-specific LPAQ desaturase lineage having Delta 11 regioselectivity, MbraKPSE-a and MbraKPSE-b, belonging to the pheromone gland-specific KPSE desaturase lineage having Delta 9 regioselectivity and a substrate preference for palmitic acid (16:0) over oleic acid (18:0), and MbraNPVE, belonging to the NPVE desaturase lineage having Delta 9 regioselectivity and a substrate preference 18:0 > 16:0. Full-length cDNAs corresponding to the two most abundantly expressed pheromone gland-specific desaturase transcripts, MbraLPAQ and MbraKPSE-b, were isolated and assayed for their ability to genetically complement the UFA auxotrophy of a desaturase-deficient ole1 strain of Saccharomyces cerevisiae. The MbraLPAQ desaturase restored UFA prototrophy and GC-MS analysis identified Z11-16:1 and Z11-18:1 as the predominant UFAs produced. Surprisingly, MbraKPSE-b failed to complement the ole1 mutation, although it shares > 98 % amino acid sequence similarity with other noctuid KPSE desaturases that do. Site-directed mutagenesis of either or both of two nonconservative amino acid substitutions restored functionality to the MbraKPSE-b protein, although GC-MS analysis revealed that neither reversion resulted in the characteristic KPSE substrate preference for 16:0.

• 出版日期2008-5