A multifunctional bioactive material that stimulates osteogenesis and promotes the vascularization bone marrow stem cells and their resistance to bacterial infection

作者：Ma, Chuang; Wei, Qin; Cao, Bo; Cheng, Xinchun; Tian, Juling; Pu, Hongwei; Yusufu, Aihemaitijiang^*; Cao, Li^*

来源：PLos One, 2017, 12(3): e0172499.

DOI：10.1371/journal.pone.0172499

• MESOPOROUS SILICA NANOPARTICLES
• GROWTH FACTOR-BB
• ANTIBACTERIAL ACTIVITY
• IN-VITRO
• ANGIOGENESIS
• GLASS
• OSTEOMYELITIS
• CEMENT
• MICROPARTICLES
• CYTOTOXICITY

The main limitation of tissue engineering lies in the inability to stimulate osteogenesis, angiogenesis of stem cells and broad-spectrum antimicrobial activity. However, the development of multifunctional bioactive materials with these capabilities remains a great challenge. In this study, we prepared mesoporous silica nanoparticles encapsulated with silver nanocrystals (AG-MSN) with uniform sphere size and mesopores. Platelet-derived growth factor BB (PDGF-BB) was effectively loaded in the AG-MSN mesopores (P-AG-MSN). The silicon ions (Si) released by P-AG-MSN stimulate osteogenic differentiation of bone marrow stromal cells (BMSC) by activating the alkaline phosphatase (ALP) activity of bone-related genes and increasing protein (OCN, RUNX2 and OPN) expression. Ag+ ions could be slowly released from the interior of the shell, highlighting their durable antibacterial activity. The sustained release of PDGF-BB from P-AG-MSN stimulated the angiogenic differentiation of BMSC, as indicated by the enhanced secretion of vascular endothelial growth factor (VEGF), HIF-1 alpha, HGF and ANG-1 and protein expression. Our results show that P-AG-MSN can clearly promote BMSC osteostimulation and vascularization. This research serves as a preliminary study of the utilization of this multifunctional mixture to fabricate a new active biological scaffold that integrates BMSC osteostimulation, vascularization and bactericidal effects by 3D printing technology.

• 出版日期2017-3-30
• 单位新疆医科大学; 新疆维吾尔自治区人民医院