Preparation of encapsulated alliinase in alginate microparticles

作者：Ko J A; Lee Y L; Jeong H J; Park H J^*

来源：Biotechnology Letters, 2012, 34(3): 515-518.

DOI：10.1007/s10529-011-0791-5

• Alliinase
• Garlic
• Encapsulation
• Microparticles

Alliinase (alkylsulphenate lyase, EC 4.4. 1.4), which catalyses the production of allicin, was immobilized in alginate microparticles. Addition of pyridoxal 5%26apos;-phosphate to the microparticles enhanced alliinase activity. Encapsulated alliinase were significantly higher (30 and 22%, respectively) than those of non-encapsulated alliinase at 60 degrees C and at pH 2. Therefore, microencapsulation of alliinase with alginate can offer an effective way of sustaining enzyme activity during oral administration and passage through the stomach.

• 出版日期2012-3