Phase angle spectroscopy on transparent conducting polymer electrodes for real-time measurement of epithelial barrier integrity

作者：Loffler S; Richter Dahlfors A^*

来源：Journal of Materials Chemistry B, 2015, 3(25): 4997-5000.

DOI：10.1039/c5tb00381d

To facilitate automated real-time monitoring of the formation or disruption of an epithelial monolayer, phase angle spectroscopy was performed in custom-designed cell culture dishes including a transparent conductive polymer-based 2-electrode device. A peak in phi amplitude at 10(3)-10(5) Hz specifically correlated to increased capacitance by proliferating cells. Dynamic monitoring of cell density, and barrier integrity during monolayer formation and disruption were investigated in combination with live-cell microscopy.

• 出版日期2015