Nucleostemin regulates proliferation and migration of gastric cancer and correlates with its malignancy

作者：Wu Hongxue; Wang Weixing^*; Tong Shilun; Wu Chong

来源：International Journal of Clinical and Experimental Medicine, 2015, 8(10): 17634-17643.

• Gastric cancer (GC)
• nucleostemin (NS)
• SGC-7901
• small interfering RNA
• apoptosis
• target therapy

Objective: The aim of this study was to investigate the effects of nucleostemin (NS) knocking down in SGC-7901 gastric cancer cell line and investigates its correlation with the metastasis and TNM stage ingastric cancer (GC) patients. Methods: NS expression was assessed using immunohistochemistry in 421 patients with GC. The correlation between NS expression, clinicopathological features and prognosis was analyzed. NS gene silencing was performed using a specific small interfering RNA (NS-siRNA). The gene expression level of NS was evaluated by PCR. The viability and growth rate of SGC-7901 cells were determined by trypan blue exclusion test. Cell cycle distribution of the cells was analyzed by flow cytometry. Results: High NS expression was correlated with node metastasis, distant metastasis and TNM stage. Kaplan-Meier survival analysis revealed that patients with low NS expression had significantly longer survival than those with high NS expression. Moreover, our results showed that NS knocking down inhibited proliferation and viability of SGC-7901 cells in a time-dependent manner. Cell cycle studies revealed that NS depletion resulted in G1 cell cycle arrest at short times of transfection (24 h) followed with apoptosis at longer times (48 and 72 h), suggest that post-G1 arrest apoptosis is occurred in SGC-7901 cells. Conclusion: Overall, these results point to essential role of NS in SGC-7901 cells, thus, this gene might be considered as a promising target for treatment of GC.

• 出版日期2015
• 单位武汉大学