Lysophosphatidic acid-activated Cl- current activity in human systemic sclerosis skin fibroblasts

作者：Yin, Zhaohong; Carbone, Laura D.; Gotoh, Mari; Postlethwaite, Arnold; Bolen, Alyssa L.; Tigyi, Gabor J.; Murakami-Murofushi, Kimiko; Watsky, Mitchell A.^*

来源：Rheumatology, 2010, 49(12): 2290-2297.

DOI：10.1093/rheumatology/keq260

• Cl- channel
• Lysophosphatidic acid
• alpha-Smooth muscle actin
• Myofibroblast
• Fibroblast

Methods. Skin biopsies were taken from involved and uninvolved skin of SSc patients and controls. Whole-cell perforated patch-clamping was used to measure IClLPA activity in fibroblasts isolated and cultured from these biopsies. Western blotting was used to measure alpha-smooth muscle actin (alpha-SMA). Proliferation was measured using a colorimetric assay.
Results. Fibroblasts cultured from SSc skin show significantly increased IClLPA activity following LPA exposure compared with control skin fibroblasts. alpha-SMA protein was significantly increased in cultured SSc skin fibroblasts vs controls. No significant differences in proliferation rates were found.
Conclusions. Elevated IClLPA activity is a hallmark of SSc skin fibroblasts. Blocking IClLPA activation may be a new therapeutic approach for treating SSc-associated fibrosis.

• 出版日期2010-12